A Novel Full-Length Transcriptome Resource for Black Tiger Shrimp (Penaeus monodon) Developed Using Isoform Sequencing (Iso-Seq)

National Science and Technology Development Agency (NSTDA)

In this study, we applied long-read Pacific Biosciences (PacBio) isoform sequencing (Iso-seq) to generate the first full-length transcriptome assembly for black tiger shrimp. Full-length mRNA sequences from nine major organs and hemocytes were obtained using PacBio's circular consensus sequencing (CCS) technology.